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Invitrogen
Collagen V (soluble) Monoclonal Antibody (1E2-E4/Col5)
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Product Details
CSI 006-01-02
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Dog,
Human,
Marsupial,
Sheep,
Pig,
Rabbit
Host/Isotype
Mouse
/ IgG2a, kappa
Class
Monoclonal
Type
Antibody
Clone
1E2-E4/Col5
Immunogen
Acid-digested pepsin soluble dog type V collagen.
Conjugate
Form
Liquid
Concentration
1.05 mg/mL
Purification
Protein A/G
Storage buffer
PBS, pH 7.4
Contains
15mM sodium azide
Storage conditions
4°C, store in dark
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_1073787
Product Specific Information
CSI 006-01-02 is highly specific for type V collagen. It has been shown to have no cross-reactivity with type I, III and VI collagens by ELISA and immunoblotting. There is no evidence for cross-reactivity with other connective tissue proteins (laminin, fibronectin, elastin). CSI 006-01-02 shows reactivity with human, dog, calf, sheep, pig and kangaroo in type V collagens by ELISA and immunoblotting. CSI 006-01-02 does not show reactivity with mouse, rat, guinea pig and chicken type V collagens by ELISA and immunoblotting.
CSI 006-01-02 has been successfully used in Western blotting, ELISA and Immunohistochemistry procedures. CSI 006-01 has been used succesfully for immunohistology on frozen unfixed sections of human, bovine and dog skin, of foetal bovine cornea, and of new dog tissue associated with a biomaterial implant. If fixation of tissue is required, acetone or ethanol is recommended. CSI 006-01 binds poorly to Collagen V when tested in ELISA with Collagen V coated directly onto the microtiter well.
NOTE: Concentration is lot-dependent and can vary from 0.85-1.15 mg/mL
Target Information
COL5A1 encodes an alpha chain for one of the lowabundance fibrillar collagens. Fibrillar collagen molecules aretrimers that can be composed of one or more types of alpha chains.Type V collagen is found in tissues containing type I collagen andappears to regulate the assembly of heterotypic fibers composed ofboth type I and type V collagen. COL5A1 product is closelyrelated to type XI collagen and it is possible that the collagenchains of types V and XI constitute a single collagen type withtissue-specific chain combinations. Mutations in this gene areassociated with Ehlers-Danlos syndrome, types I and II.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: AB collagen; alpha-1 type V collagen; COL5; Collagen 5; Collagen alpha-1(V) chain; collagen type 5 alpha 1; collagen, type V, alpha 1; procollagen alpha 1(V); RP11-263F14.1; Type V Collagen
Gene Aliases: COL5A1; EDSC
UniProt ID: (Human) P20908
Entrez Gene ID: (Pig) 397533, (Sheep) 101107777, (Dog) 480684, (Rabbit) 100327268, (Human) 1289
blood vessel development
heart morphogenesis
cell adhesion
cell migration
extracellular matrix organization
collagen fibril organization
collagen catabolic process
collagen biosynthetic process
wound healing, spreading of epidermal cells
tendon development
extracellular fibril organization
skin development
integrin biosynthetic process
eye morphogenesis
regulation of cellular component organization
negative regulation of endodermal cell differentiation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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